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Identification of causative species in patients with cuteneous leishmaniasis in Diyarbakır by polymerase chain reaction pcr restriction fragment lenght polymorphism RFLP   
Yazarlar (7)
Nezahat Akpolat
Dicle Üniversitesi, Türkiye
Doç. Dr. İbrahim Halil YILDIRIM Doç. Dr. İbrahim Halil YILDIRIM
Dicle Üniversitesi, Türkiye
Prof. Dr. Muttalip ÇİÇEK Prof. Dr. Muttalip ÇİÇEK
Dicle Üniversitesi, Türkiye
Şebnem Nergiz Öztürk
Dicle Üniversitesi, Türkiye
Özgür Ezin
Nida Özcan
Dicle Üniversitesi, Türkiye
Mehmet Harman
Dicle Üniversitesi, Türkiye
Devamını Göster
Özet
Background: Leishmaniasis is a protozoan disease caused by more than twenty Leishmania species which are transmitted by infected phlebotomine sandflies to humans. Leishmaniasis include multiple clinical syndromes such as visceral , mucosal and cutaneous forms. Cutaneous Leishmaniasis (CL) is the most common and endemic form in southeastern Turkey. Purpose: CL can be caused by L. major, L. tropica, L. mexicana and L. amazonensis. Microscopy remains to be the standard diagnostic method because of its cheapness, ease of application and high specificity . Microscopic examination of smears, parasite culture and serological tests are performed for diagnosis. Recently several molecular methods, especially those based on the Polymerase chain reaction (PCR) have been developed for identification of Leishmania species. Method: In this study, 150 smear samples taken from patients with clinical findings of CL were studied by PCR- RFLP (restriction fragment length polymorphism) Results: Leishmania tropica was detected in 128 smear sample. L. tropica was found to be the most common species causing CL in southeastern Turkey. Conclusion: Identification of Leishmania species is important because different types may require different treatments. Determining the common species in the region may lead to develop treatment protocols.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale
Dergi Adı International Archives of Medical Research
Dergi Tarandığı Indeksler GOOGLE SCHOOL
Makale Dili İngilizce
Basım Tarihi 01-2014
Cilt No 6
Sayı 2
Sayfalar 17 / 20
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Google Scholar 2

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