| Yazarlar (2) |
Dr. Öğr. Üyesi Mevlüde Alev ATEŞ
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Seher Karaman Erkul
Aksaray Üniversitesi, Türkiye |
| Özet |
| Molecular regions of DNA were often used to clarify the genetic relationships of plants in recent years. Particularly, the ITS (Internal Transcribed Spacer-ITS1+ ITS2) region was mostly suggested for molecular phylogenetic studies due to its highly repeated in number in plant genomes and large copy numbers that support PCR amplification. At all taxonomic levels, ITS2 indicates significant differentiation among species; so, it is used as an important marker in molecular systematics studies. Furthermore, not only primary sequences also secondary structure of ITS2 region became valuable in species divergence and was used as a molecular morphological character. In the current study, different representative Astragalus species of different sections from Turkey were used as samples. The ITS2 regions of the samples, which were approximately 220bp in length, were analyzed. Sequences were retrieved from NCBI data bank and sequences were aligned using ClustalW and Kimura-2 parameter to calculate the genetic distances. Phylogenetic tree was also constructed with Maximum Likelihood method with Kimura-2 parameter additionally with G (gamma) distribution at MEGA X software. Moreover, secondary structures of species were predicted via the tools from the ITS2 database (http://its2. bioapps. biozentrum. uni-wuerzburg. de/) and mFOLD web server. According to the results, it was seen that both the overall genetic distances and the phylogenetic tree indicated section separations of Astragalus genus, and also ITS2 secondary structure predictions were represented these genetic differences visibly like a morphological character. For instance … |
| Anahtar Kelimeler |
| Bildiri Türü | Tebliğ/Bildiri |
| Bildiri Alt Türü | Tam Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum) |
| Bildiri Niteliği | Alanında Hakemli Uluslararası Kongre/Sempozyum |
| Bildiri Dili | İngilizce |
| Kongre Adı | AHI EVRAN International Conference on Scientific Research |
| Kongre Tarihi | 30-11-2021 / 02-12-2021 |
| Basıldığı Ülke | Türkiye |
| Basıldığı Şehir | Kırşehir |
