Secondary Structure Form of ITS2 Region: A Significant Labeling Tool at all Taxonomic Levels
Yazarlar (6)
Doç. Dr. Mevlüde Alev ATEŞ Kırşehir Ahi Evran Üniversitesi, Türkiye
Seher Karaman Aksaray Üniversitesi, Türkiye
Dudu Özlem Mavi İdman
Türkiye
Selçuk Tuğrul Körüklü Ankara Üniversitesi, Türkiye
Barış Bani Kastamonu Üniversitesi, Türkiye
Makale Türü Özgün Makale (Diğer hakemli uluslarası dergilerde yayınlanan tam makale)
Dergi Adı Biodiversity Studies
Dergi ISSN 2791-7347
Dergi Tarandığı Indeksler
Makale Dili İngilizce Basım Tarihi 12-2022
Cilt / Sayı / Sayfa 1 / 2 / 37–43 DOI 10.56494/dnbgt.2022.6
Makale Linki https://www.biodiversitystudies.org/index.php
UAK Araştırma Alanları
Bitki Sistematiği Genetik Moleküler Biyoloji
Özet
DNA barcoding techniques are the most popular methods for plant sciences in recent years for species identifications (CBOL Plant working group, 2009) due to its non affectiveness property by external factors or development stage. Additionally, the main material of the DNA method can be easily isolated from all tissues (Sucher and Carles, 2008; Seethapathy et al., 2015; Wu et al., 2015; Mishra et al., 2016). Hence this provides a powerful method for species identifications at all levels (Yu et al., 2017). The important advantage of barcoding techniques is the usage of short sequences of standart part of the genome with respect to whole genome to detect the identity of samples. Especially rbcl and matK plastid coding genes which are the parts of chloroplast genome have been adviced as the most useful barcodes due to their ability at amplification and sequencing processes by CBOL Plant Working Group (2009)(Michel et al., 2016; Mohamed, 2016; Al-Juhani and Khalik, 2021). In recent plant phylogenetic studies, the internal transcribed spacer (ITS) region of rDNA is used as complementary for matK and rbcl regions with their relatively strong discrimination property (CPBG China Plant BOL Group, 2011). NrDNA ITS region is composed of 2 different intergenic regions with highly conserved 5.8
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