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Improved in vitro propagation and direct acclimatization of Cryptocoryne wendtii in aquarium in the presence of aquarium fish Puntius tetrazona (Bleeker)    
Yazarlar
Sündüs Ünal
Gürel Türkmen
Ege Üniversitesi, Türkiye
Bülent Yağmur
Ege Üniversitesi, Türkiye
Doç. Dr. Meltem BAYRAKTAR
Kırşehir Ahi Evran Üniversitesi, Türkiye
Aynur Gürel
Ege Üniversitesi, Türkiye
Özet
Cryptocoryne wendtii is an important ornamental aquatic plant and has difficulties in conventional cultivation. In the present study, we propose an efficient in vitro propagation and acclimatization protocols for C. wendtii. The highest number of shoots was achieved on Murashige and Skoog (MS) medium containing 4.0 mg/L BA+1.0 mg/L IBA. In the second subculture, the number of shoots per explant increased from 7.2 to 51.8 in this medium. The shoot length per explant, the percentage of shoots rooted, and the number of roots per shoot were maximum in MS medium supplemented with 1.0 mg/L IBA. The in vitro rootless and rooted shoots were acclimatized in aquariums containing different substrates [Calcite (Ca), River Sand (RS), Zeolite (Ze) and Shell Grit (SG)] and tetrazona fish Puntius tetrazona (Bleeker). One third of the water in the aquariums was replaced with fresh tap water every two weeks and the water analyses were carried out before replacement of aquarium water everytime. All shoots were successfully acclimatized for a period of 3 months. The RS and rooted shoot explants were found to be more effective than the other substrates, and rootless shoots in terms of all parameters that were tested.
Anahtar Kelimeler
Calcite, Ornamental aquatic plants, River sand, Shell grit, Wendt's cryptocoryne, Zeolite
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY
Dergi ISSN 0019-5189
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 05-2019
Cilt No 57
Sayfalar 330 / 337
Makale Linki http://nopr.niscair.res.in/handle/123456789/47168
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 2
Google Scholar 5

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