Enhancement of stevioside production by using biotechnological approach in in vitro culture of Stevia rebaudiana
Yazarlar (7)
Doç. Dr. Meltem BAYRAKTAR Kırşehir Ahi Evran Üniversitesi, Türkiye
Elmira Naziri
Ege Üniversitesi
Ege Üniversitesi
Fatih Karabey
Ege Üniversitesi
Ege Üniversitesi
İsmail Hakkı Akgün Ege Üniversitesi, Türkiye
Erdal Bedir İzmir Yüksek Teknoloji Enstitüsü, Türkiye
Rana Okuyucu Manisa Celâl Bayar Üniversitesi, Türkiye
Aynur Gürel Ege Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale)
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| Dergi Adı | International Journal of Secondary Metabolite | ||
| Dergi ISSN | 2148-6905 Scopus Dergi | ||
| Dergi Tarandığı Indeksler | TR DİZİN | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2018 |
| Cilt / Sayı / Sayfa | 5 / 4 / 362–374 | DOI | 10.21448/ijsm.496724 |
| Makale Linki | http://dergipark.gov.tr/doi/10.21448/ijsm.496724 | ||
| Özet |
| Stevia rebaudiana Bertoni, which is an important plant for the food and health sector, contains calorie-free natural sweet-tasting steviol glycosides (SGs). In the present study, the effects of different elicitors [methyl jasmonate (MeJA), salicylic acid (SA), or chitosan (CHI)] on the in vitro production of stevioside and rebaudioside A were carried out. For this purpose, 3-week-old in vitro plantlets were transferred into 250 mL flasks containing liquid woody plant medium (WPM) supplemented with MeJA, SA, or CHI at different concentrations (0, 50, 100, or 200 µM), and were exposed to these elicitors for 2 weeks. A HPLC method was developed to quantify the aforementioned SGs in the cultivated plantlets and all of the elicitor types and concentrations resulted in an increase in stevioside production ranged between 2.87 mg/g dry weight (DW) (Control) and 50.07 mg/g DW (100 µM MeJA). The highest number of shoot, node, leaf, leaf length, and biomass accumulation and shoot length were observed with application of 100 µM CHI and control, respectively. The present findings open new perspectives for increasing the stevioside production using a plant tissue culture system. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 38 |