| Yazarlar |
Münire Ekmekçigil
|
Doç. Dr. Meltem BAYRAKTAR
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Özge Akkuş
Muğla Sıtkı Koçman Üniversitesi, Türkiye |
|
Aynur Gürel
Ege Üniversitesi, Türkiye |
| Özet |
| An efficient in vitro mass propagation through protocorm-like bodies (PLBs) was established in Cattleya forbesii Lindl., a commercially important orchid. Whole PLBs (W-PLB) and transverse thin cell layers of PLB (tTCL-PLB) explants were cultured in RITA® bioreactors based on temporary immersion system. Explants were transferred in semi-solid or RITA® bioreactor for protocorm production or shoot regeneration. The effect of different immersion frequencies, medium volumes and inoculum densities were studied and optimized. RITA® bioreactor cultures were found to be superior compared with semi-solid cultures regarding PLB production and shoot regeneration. tTCL-PLB explant types cultured in the RITA® bioreactor with immersion for 1 min/4 h, 250 mL of medium and 20 explants showed the highest number of PLBs per RITA® (2237 PLBs) and per explant (111.9 PLBs). The highest number of PLBs per explant was 21 times higher than those from semi-solid culture. The highest number of shoots per RITA® (3998 shoots) and per explant (199.9 shoots) were observed on tTCL-PLB cultured in RITA® bioreactor (1 min/4 h; 150 mL of medium and 20 explants). The highest number of shoots per explant was 95 times higher than those grown on semi-solid culture. Mass propagation of PLBs and shoots of C. forbesii Lindl. using combined thin cell layer and RITA® temporary immersion has been adapted in commercial practice. |
| Anahtar Kelimeler |
| Bioreactor | Cattleya forbesii Lindl | In vitro | Mass propagation | Protocorm | Temporary immersion system | Thin cell layer |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | PLANT CELL TISSUE AND ORGAN CULTURE |
| Dergi ISSN | 0167-6857 |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-2019 |
| Cilt No | 136 |
| Sayı | 3 |
| Sayfalar | 451 / 464 |
| Doi Numarası | 10.1007/s11240-018-1526-2 |
| Makale Linki | http://link.springer.com/10.1007/s11240-018-1526-2 |
| Atıf Sayıları | |
| WoS | 3 |
| SCOPUS | 20 |
| Google Scholar | 31 |
