Direct plant regeneration from different explants through micropropagation and determination of secondary metabolites in the critically endangered endemic Rhaponticoides mykalea
Yazarlar (4)
S. Hayta Ege University Faculty Of Engineering, Türkiye
Doç. Dr. Meltem BAYRAKTAR Kırşehir Ahi Evran Üniversitesi, Türkiye
S. Baykan Erel Ege Üniversitesi, Türkiye
A. Gurel Ege University Faculty Of Engineering, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Plant Biosystems
Dergi ISSN 1126-3504 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 07-2017
Cilt / Sayı / Sayfa 151 / 1 / 20–28 DOI 10.1080/11263504.2015.1057267
Makale Linki http://www.tandfonline.com/doi/full/10.1080/11263504.2015.1057267
Özet
Direct plant regeneration from different explants, micropropagation and determination of secondary metabolites were studied in the critically endangered endemic Rhaponticoides mykalea (Hub.-Mor.) M.V. Agab & Greuter. Seed germination was achieved by damaging the seed coat and cultivating the embryos on Woody Plant Medium (WPM), of which 40% germinated. The epicotyls and cotyledonary petioles of seedlings were used as initial explants and direct shoot regeneration was obtained on WPM containing 2.22 μM 6-benzyladenine (BA). WPM medium supplemented with 2.22 μM BA and 4.92 μM indole-3-butyric acid (IBA) significantly improved the production of multiple shoots, resulting in an average of 5.6 shoots per explants. The highest rooting of shoots (35.6%) was observed with WPM medium containing 19.68 μM IBA with 990 μM putrescine. Plantlets with well-developed roots were transferred to soil …
Anahtar Kelimeler
Chlorogenic acid | Endemic plant | HPLC | In vitro germination | Micropropagation | Quercetin | Rhaponticoides mykalea | Scutellarin | Seed dormancy