Yazarlar (2) |
![]() Ankara Üniversitesi, Türkiye |
![]() Ahi Evran Üniversitesi, Türkiye |
Özet |
Th e genetic diversity of Pyrenophora graminea, the causal agent of barley leaf stripe disease, was characterized by restriction fragment length polymorphism (RFLP) of the rDNA internal transcribed spacer region and intersimple sequence repeat (ISSR) analysis. A total of 45 P. graminea isolates recovered from diff erent geographic origins and barley cultivars or genotypes produced identical RFLP banding patterns with HinfI, MspI, Hin6I and BsuRI restriction enzymes. ISSR analysis indicated that there was little genetic variability among the isolates. A dendrogram based on Jaccard s coeffi cient separated the isolates into 4 clusters with 12% dissimilarity. In the dendrogram, the isolates collected from diff erent geographic regions in Turkey were clustered separately from the Italian isolates. Th ese results indicated that the Turkish isolates of P. graminea were genetically homogeneous and may be derived from a single gene pool. In addition, 13 isolates were tested for pathogenic variability on 48 barley cultivars. Th e cultivars Durusu, Balkan 96 (Igri), Çumra 2001 and Anadolu 98 were found to be resistant to all leaf stripe isolates. |
Anahtar Kelimeler |
Barley (Hordeum spp.) | barley leaf stripe | genetic resistance | ISSR | PCR-RFLP |
Makale Türü | Özgün Makale |
Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
Dergi Adı | TURKISH JOURNAL OF AGRICULTURE AND FORESTRY |
Dergi ISSN | 1300-011X Wos Dergi Scopus Dergi |
Dergi Tarandığı Indeksler | SCI-Expanded |
Makale Dili | İngilizce |
Basım Tarihi | 01-2012 |
Cilt No | 36 |
Sayı | 3 |
Sayfalar | 329 / 339 |
Doi Numarası | 10.3906/tar-1012-1645 |