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Genetic characterization of Pyrenophora graminea isolates and the reactions of some barley cultivars to leaf stripe disease under greenhouse conditions       
Yazarlar (2)
Harun Bayraktar
Ankara Üniversitesi, Türkiye
Doç. Dr. Kadir AKAN Doç. Dr. Kadir AKAN
Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
Th e genetic diversity of Pyrenophora graminea, the causal agent of barley leaf stripe disease, was characterized by restriction fragment length polymorphism (RFLP) of the rDNA internal transcribed spacer region and intersimple sequence repeat (ISSR) analysis. A total of 45 P. graminea isolates recovered from diff erent geographic origins and barley cultivars or genotypes produced identical RFLP banding patterns with HinfI, MspI, Hin6I and BsuRI restriction enzymes. ISSR analysis indicated that there was little genetic variability among the isolates. A dendrogram based on Jaccard s coeffi cient separated the isolates into 4 clusters with 12% dissimilarity. In the dendrogram, the isolates collected from diff erent geographic regions in Turkey were clustered separately from the Italian isolates. Th ese results indicated that the Turkish isolates of P. graminea were genetically homogeneous and may be derived from a single gene pool. In addition, 13 isolates were tested for pathogenic variability on 48 barley cultivars. Th e cultivars Durusu, Balkan 96 (Igri), Çumra 2001 and Anadolu 98 were found to be resistant to all leaf stripe isolates.
Anahtar Kelimeler
Barley (Hordeum spp.) | barley leaf stripe | genetic resistance | ISSR | PCR-RFLP
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
Dergi ISSN 1300-011X Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2012
Cilt No 36
Sayı 3
Sayfalar 329 / 339
Doi Numarası 10.3906/tar-1012-1645