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An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat      
Yazarlar (7)
Semra Hasançebi
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, Türkiye
Zafer Mert
Field Crop Research Institute, Türkiye
Fahriye Ertuğrul
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, Türkiye
Doç. Dr. Kadir AKAN Doç. Dr. Kadir AKAN
Field Crop Research Institute, Türkiye
Yıldız Aydın
Marmara Üniversitesi, Türkiye
Funda Şentürk Akfırat
Gebze Teknik Üniversitesi, Türkiye
Ahu Altınkut Uncuoğlu
Marmara Üniversitesi, Türkiye
Devamını Göster
Özet
EST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) x ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the susceptible parent and the susceptible bulk. To investigate its association with Yr genes, 20 individuals of NILs were also amplified with BU099658 and the 206 bp marker fragment was obtained only in Yr1/6 x Avocet S. Additionally, bu099658 was screened on 65 genotypes which possessed different Yr genes/gene combination(s) and Yr1. The results indicate a close linkage of bu099658 with the Yr1 gene.
Anahtar Kelimeler
Bulk Segregant Analysis (BSA) | Marker-Assisted Selection (MAS) | Puccinia striiformis f.sp tritici | Triticum aestivum L. | Yr1
Makale Türü Açık Erişim Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Czech Journal of Genetics and Plant Breeding
Dergi ISSN 1212-1975 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2014
Cilt No 50
Sayı 1
Sayfalar 11 / 18
Doi Numarası 10.17221/109/2013-cjgpb
Makale Linki http://www.agriculturejournals.cz/web/cjgpb.htm?volume=50firstPage=11type=publishedArticle