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Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)      
Yazarlar (10)
Handan Balta
Istanbul Üniversitesi, Türkiye
Özge Karakaş Metin
Tubıtak Marmara Research Center, Türkiye
Funda Akfirat Şentürk
Gebze Teknik Üniversitesi, Türkiye
Fahriye Ertuǧrul
Agriculture Forestry And Veterinary Research Group, Türkiye
Semra Hasançebi
Tubıtak Marmara Research Center, Türkiye
Yildiz Aydin
Marmara Üniversitesi, Türkiye
Doç. Dr. Kadir AKAN Doç. Dr. Kadir AKAN
Field Crop Research Institute, Türkiye
Zafer Mert
Field Crop Research Institute, Türkiye
Müge Türet
Boğaziçi Üniversitesi, Türkiye
Ahu Altinkut Uncuoǧlu
Marmara Üniversitesi, Türkiye
Devamını Göster
Özet
An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F 2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F 2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC (133bp) marker by the PCR walking method to design SCAR markers for wheat breeding programs.
Anahtar Kelimeler
AFLP | bread wheat | disease resistance | SCAR | yellow rust
Makale Türü Açık Erişim Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Turkish Journal of Biology
Dergi ISSN 1300-0152 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2014
Cilt No 38
Sayı 3
Sayfalar 371 / 379
Doi Numarası 10.3906/biy-1311-8
Makale Linki http://journals.tubitak.gov.tr/biology/issues/biy-14-38-3/biy-38-3-8-1311-8.pdf