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Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)      
Yazarlar (10)
Handan Balta
Özge Karakaş Yıldırım
Afyon Kocatepe Üniversitesi, Türkiye
Funda Şentürk Akfırat
Gebze Teknik Üniversitesi, Türkiye
Fahriye Ertuğrul
Semra Hasançebi
Trakya Üniversitesi, Türkiye
Yıldız Aydın
Doç. Dr. Kadir AKAN Doç. Dr. Kadir AKAN
Ahi Evran Üniversitesi, Türkiye
Zafer Mert
Müge Türet
Boğaziçi Üniversitesi, Türkiye
Ahu Altınkut Uncuoğlu
Marmara Üniversitesi, Türkiye
Devamını Göster
Özet
An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F 2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F 2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC (133bp) marker by the PCR walking method to design SCAR markers for wheat breeding programs.
Anahtar Kelimeler
AFLP | bread wheat | disease resistance | SCAR | yellow rust
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı TURKISH JOURNAL OF BIOLOGY
Dergi ISSN 1300-0152 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2014
Cilt No 38
Sayı 3
Sayfalar 371 / 379
Doi Numarası 10.3906/biy-1311-8
Makale Linki http://journals.tubitak.gov.tr/biology/issues/biy-14-38-3/biy-38-3-8-1311-8.pdf