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Isolation and Characterization of Rhizobium Strains from Wild VetchCollected from High Altitudes in Erzurum Turkey    
Yazarlar
Ahmet Adiguzel
Prof. Dr. Hatice ÖĞÜTCÜ Prof. Dr. Hatice ÖĞÜTCÜ
Kırşehir Ahi Evran Üniversitesi, Türkiye
Ozlem Baris
Mehmet Karadayi
Medine Gulluce
Fikrettin Sahin
Özet
Recently, there has been a growing level of interest in environmental friendly sustainable agricultural practices and organic farming systems. Increasing and extending the role of biofertilizers such as Rhizobium would decrease the need for chemical fertilizers and reduce adverse environmental effects. Thus, in the development and implementation of sustainable agriculture techniques, biofertilization is of big importance in alleviating the deterioration of natural and environmental pollution. Besides, the assessment of rhizobial genetic diversity is contributing both to the worldwide knowledge of the biodiversity of soil microorganisms and to the utility of rhizobial collections. Particularly, in the last decades, the use of molecular techniques has been contributed greatly to enhance the knowledge of rhizobial diversity. This study was conducted in order to determine the phenotypic and genotypic characterization of Rhizobium leguminosarum subsp. viciae strains that were isolaled from perennial wild vetch (Vicia cracca) collected from high attitudes (2000-2500 m) in mountains of Erzurum, Eastern Anatolia, Turkey. In this work, rep-PCR (ERIC-, REP- and BOX-PCR) fingerprinting method was used for the genotypic characterization of R. leguminosarum subsp. viciae strains isolated from perennial wild vetch. As a result, a high intraspecies diversity was observed in the rep-PCR (ERIC-, REP- and BOX-PCR) analysis with BOX, ERIC and REP primers between R. leguminosarum subsp. viciae strains.
Anahtar Kelimeler
Rhizobium leguminosarum subsp viciae | phenotypic characterization | genotypic characterization | REP-PCR | ERIC-PCR | BOX-PCR
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı ROMANIAN BIOTECHNOLOGICAL LETTERS
Dergi ISSN 1224-5984
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2010
Cilt No 15
Sayı 1
Sayfalar 5017 / 5024
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 6

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