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GENETIC CHARACTERIZATION OF PEPPER (Capsicum annuum L.) GENOTYPES FROM CENTRAL ANATOLIA WITH SSR AND SCAR MARKERS     
Yazarlar
 Hakan BAŞAK Hakan BAŞAK
Türkiye
Gölge Sarıkamış
Türkiye
Gamze Çakırer Seyrek
Türkiye
Dr. Öğr. Üyesi Mevlüde Alev ATEŞ Dr. Öğr. Üyesi Mevlüde Alev ATEŞ
Kırşehir Ahi Evran Üniversitesi, Türkiye
Özet
The major objective in pepper breeding programs is to generate high yielding novel varieties resistant to pests and diseases, tolerant to abiotic stress conditions with improved fruit quality traits including capsaicin content. Germplasm collections are important sources of variability for breeding studies. Molecular markers are important tools to evaluate genetic relationships among germplasm collections. Moreover, markers are used to select the genotypes conferring the desired traits via marker-assisted selection (MAS) as a powerful approach accelerating breeding programs. In the current study, 56 pepper genotypes selected among 313 pepper genotypes collected from Kirrehir province in the Central Anatolian region of Turkey according to their agronomic and morphological characteristics were used for molecular assays. Six SSR markers two of which were linked to fruit morphology were selected to characterize pepper genotypes according to their high polymorphism information content. Three SCAR markers associated with capsaicinoid synthesis and resistance to Phytophtora capsici (Phyto.5.2) in pepper were used to assess pungency and resistance among genotypes. According to the results obtained with SSR markers, the total number of alleles ranged from 1 to 8 among genotypes. The most polymorphic SSR markers were CaeMS015 and CAMS452 within the pepper population. The genetic distance among genotypes was determined ranging between 0.75-1.00. The segregation of the SCAR marker BF6-BF8 linked to pungency in pepper was determined relative to pungent and sweet reference cultivars. The OP004.717 SCAR marker linked to Phytophtora capsici was tested among genotypes relative to CM334 pepper variety, known as a source of resistance to Phytophtora.
Anahtar Kelimeler
Capsicum annuum | Capsaicin | Phytophtora capsici | SSR | SCAR
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı GENETIKA-BELGRADE
Dergi ISSN 0534-0012
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 01-2022
Cilt No 54
Sayı 3
Sayfalar 1171 / 1182
Doi Numarası 10.2298/GENSR2203171B
Makale Linki https://www.dgsgenetika.org.rs/publications/journals-genetika/