| Proje Ekibi |
Prof. Dr. Serap YALÇIN AZARKAN
Kırşehir Ahi Evran Üniversitesi, Türkiye Projedeki Rolü: Yürütücü |
| Proje Türü | (Ulusal) Yükseköğretim Kurumları Tarafından Destekli Bilimsel Araştırma Projesi |
| Proje No | - |
| Proje Konusu | Abstract Background: This study investigated the preparation, characterization and antiproliferative effect of 5- Fluorouracil loaded PHB coated magnetic nanoparticles for breast cancer therapy. Materials and Methods: In this study, we developed a new method that enabled for the first time in situ and PHB coated MNPs, involving the coprecipitation of iron salts in the presence of PHB. The structural properties, functional groups, size distribution and magnetic properties of the synthesized nanoparticles were characterized by XRD, TGA, FT-IR, TEM, VSM, and DLS analyses. The 5- Fluorouracil loaded PHB-MNPs was confirmed by FTIR analysis. Drug loading and release characteristics, and stability of the nanoparticles were investigated. In vitro cytotoxicity effects of 5- Fluorouracil-loaded PHB-MNPs on SKBR-3 cells were evaluated by means of the XTT Cell Proliferation Kit (Biological Industries, Israel) according to manufacturer's instructions. SKBR-3 cells were seeded into 96-well microtiter plates (Greiner) at a concentration of 5.0X104. Results: The cytotoxic effect of 5- Fluorouracil -loaded PHB-MNPs on SKBR-3 cells was investigated by XTT cell proliferation assay, and IC50 values were calculated. In this study, empty PHB-MNPs were found not significantly cytotoxic up to 500 mg/mL. The IC50 value of 600 mg/mL 5- Fluorouracil loaded PHB-MNPs were found as 12,5 μM on SKBR-3 cells. IC50 value of free 5- Fluorouracil as 23,7 μM for SKBR-3 cells. From the IC50 values, it was seen that the loading of 5- Fluorouracil onto PHB-MNPs increased its efficiency nearly up to 3- to 3.5-fold. Conclusion: In this study, PHB magnetic nanoparticles were prepared for targeted delivery of 5- Fluorouracil. The results indicated that the synthesized magnetic nanoparticles have a high potential to be used as a 5- Fluorouracil delivery system, which can be targeted to tumor cells under a magnetic field. The magnetic targeting will prevent the side effects of chemotherapy, while the effective release from the nanoparticles may reduce the amount of 5- Fluorouracil required, and the frequency of drug administration. |
| Proje Durumu | Tamamlandı |
| Başlangıç Tarihi | 04-05-2017 |
| Bitiş Tarihi | 04-10-2017 |
